The following microscopes are available:

AF7000 Widefield Fluorescence Microscope - live (Room 00.431)

The AF7000 (Leica) is an inverted widefield microscope with fluorescence (UV, green, red, far red fluorescence) and diverse contrast methods in transmitted light (brightfield, phase contrast, DIC, polarisation). It is equipped with an environmental control box (temperature, CO2) for live cell imaging and a software module for small screening applications. The following objectives are available: 1.25x/0.04 dry, 10x/0.3 dry, 20x/0.4 dry or 20x/0.8 dry, 40x/0.6 dry, 63x/1.4 oil, 63x/1.2 water, 100x/1.4 oil.

Thunder Widefield Fluorescence Microscope - live (Room -1.291)

The THUNDER (Leica) is an inverted widefield microscope with fluorescence and built in image processing tools for background subtraction and adaptive deconvolution (based on Leica LIGHTNING).

Diverse contrast methods in transmitted light (brightfield, phase contrast, DIC) are available.

It is equipped with an environmental control box (temperature, CO2) for live cell imaging and a software module for small screening applications. The following objectives are available: 10x/0.3 dry, 25x/0.95 water (long working distance 2.5 mm), 40x/0.95 air, 63x/1.4 oil, 100x/1.44 oil.

There are also 8 LEDs for illumination: 395, 438, 475, 511, 555, 575, 635 and 730 nm inclusive, with corresponding filter sets.

IncuCyte SX5 Widefield Fluorescence Screening Microscope - live (Room 00.281)

The IncuCyte SX5 (Sartorius) is a box-type widefield screening microscope for live cell imaging with many predefined assays and analysis pipelines. The set-up is equipped with 4x, 10x and 20x air objectives and located in an incubator chamber (temperature, CO2) ideally for long-term live cell imaging. There are six places for multiwell plates (6, 12, 24, 48 or 96 well) and/or four places for chambered slides.

Two fluorescence modules are available:

PH/G/R: Phase contrast/green (441-481 nm, 503-544 nm)/red (567-607 nm, 622-704 nm) 

PH/G/O/NIR: Phase contrast/green (453-485 nm, 494-533 nm)/orange (546-568 nm, 576-639 nm)/NIR (648-674 nm, 685-756 nm)

CX-F 3D Cell Explorer - live (Room -1.291)

The 3D Cell Explorer fluo (nanolive) is a holo-tomographic widefield microscope with fluorescence options for gentle live cell imaging. The contrast is based on refractive index measurements in living samples and provides "label-free" discrimination of several organelles (mitochondria, lipid vesicles, nucleus, nucleoli, lysosomes, etc.).

Whole 3D volumes can be imaged within 2 sec (80 µm x 80 µm x 30 µm). The set-up is equipped with a 60x air objective and an incubator chamber (temperature, CO2) for live cell imaging. Fluorescence options (widefield) for green, red and far-red fluorophores are available.

SPE Confocal Microscope (Room 00.461)

This is an upright confocal microscope with one detector, three objectives (10x/0.3, 40x/1.15 oil, 63x/1.3 oil) and four laser lines (405, 458, 561, 635 nm) 

SP5 Confocal Laser Scanning Microscope (Room 00.461)

The TCS SP5 (Leica) is an inverse confocal fluorescence microscope optimised for imaging fixed samples with multicolour fluorescence. Four lasers available for excitation (405, argon (458, 476, 488, 495, 514), 561 and 633 nm). The fluorescence emission can be continuously adjusted between 400-800 nm and is detected by 4 PMTs. Mosaic scanning of large objects is possible due to the motorised xy-table. The following objectives are available: 10x/0.3 dry, 20x/0.7 dry, 40x/1.3 oil, 63x/1.2 water, 63x/1.4 oil.

SP5 STED CW Super-Resolution Microscope (Room -1.261)

The TCS SP5 STED CW (Leica) confocal microscope is dedicated to super-resolution microscopy based on STED (STimulated Emission Depletion) and live cell imaging. Super-resolution in the xy-direction (ca. 70 nm) is achieved for fluorophores with emission spectra overlapping with the 592 nm depletion laser. Additionally, the setup is equipped with a FCS module (Picoquant) and APD sensors to measure diffusion. Three lasers with the following wavelengths are available for excitation: argon (458, 476, 488, 495, 514), 561 and 633 nm. The emission can be continuously adjusted between 400-800 nm and is detected by 3 PMTs and 2 HyDs. The following objectives are available: 10x/0.3 dry, 20x/0.7 immersion, 40x/1.1 water, 63x/1.2 water, 63x/1.4 oil, 100x/1.4 oil.

GSDIM Super-Resolution Microscope (Room -1.261)

The GSD 3D (Leica) widefield microscope is dedicated to super-resolution microscopy based on fluorophore blinking achieved by GSD (Ground State Depletion) and the single molecule localisation algorithm (also called dSTORM or PALM). Structures in the range of 40 nm (xy) and 50 nm (z) can be resolved. The microscope is further equipped with a TIRF (total internal reflection) module, which is ideal for imaging samples located close to the cover glass/water interface, such as membrane receptors. The objective for super-resolution and TIRF is 160x/1.47 oil.

Opera Phenix Spinning Disk Confocal Microscope – live (Room 00.531)

The Opera Phenix (Perkin Elmer) is a high-content screening (HCS) microscope system, built to handle multi-well plates as well as slides. The system can be operated in widefield mode and in spinning disc confocal mode. Fixed and living cells/organisms can be recorded. The system is equipped with two sCMOS cameras for fast recording, with four laser lines (405, 488, 561 and 640 nm) and the following air objectives: 1.25x (0.03 NA), 10x (0.3 NA). Available water immersion objectives are: 20x (1.0 NA), 40x (1.1 NA) and 63x (1.15 NA).

VisiScope Spinning Disk Confocal Microscope – live (Room -1.261)

The Spinning Disc Confocal (5 Elements, Visitron) microscope has an ablation laser on a Nikon stand with a CSU-W1 spinning disk (25µm and 50 µm pinholes) from Yokogawa. It is equipped with two sCMOS cameras for simultaneous live cell imaging in two channels and seven laser lines. The microscope is optimised for live cell imaging with TIRF, FRAP and DNA ablation options (355 nm pulsed laser). The following objectives are available: 10x (0.45 NA, air), 10x (0.5 NA, gly/water/oil), 20x (0.75 NA, air), 40x (1.25 NA, water), 60x (1.2 NA, water), 100x (1.49 NA oil).

M80 Demonstration Stereo Microscope (Room 00.453)

Equipped with an LED goose neck, a Rottermann contrast transmission light base and a camera to show others via a 24“ monitor how to dissect/prepare specimen under the M80.

M205 Fluorescence Stereo Microscope (Room 00.453)

Equipped with a camera, a fluorescence light source and three filters for UV, green and red fluorophores. 

DM2500 Fluorescence Upright Microscope (Room 00.453)

Equipped with a colour camera, a fluorescence light source and three filters for UV, green and red fluorophores. This microscope has 5 objectives (2.5x/0.07, 10x/0.25, 20x/0.4, 40x/0.65, 63x/0.95), all of which are air objectives! This is a specialised microscope for H&E staining with brightfield illumination. No DIC or phase contrast is available 

4 Image Processing Stations (Room 00.457)

Commercial (Imaris, vision 4D, VisiView, LAS-X, Zen blue Intellesis, Huygens and Huygens Remote Manager, Harmony, Columbus, GraphPad Prism, MatLab) and open source software tools are available (e.g. Fiji (ImageJ), QuPath, Icy).

 

For all microscopes and different software you can find user manuals here.